Rotaviruses

Содержание

1. Rotaviruses
2. Слайд 2
3. MorphologyFamily Reoviridae 70-85 nm diameter Nearly spherical
4. Genome11 segments of double-stranded RNA structural
5. Genome
6. Classificationdefined by cross-neutralization with polyclonal
7. ClassificationDistribution of rotavirus strains from a global
8. ReplicationAttached to cell receptors contained sialic acidInternalized
9. ReplicationSecondary transcription occurs in cytoplasm in later
10. Replication
11. Pathogenesisinfect upper two-third of duodenal epithelial cell
12. Pathogenesis
13. EpidemiologyPrimary transmission mode is fecal-oralingestion
14. EpidemiologyRotavirus causes the highest level of diarrhea among developed and developing countries
15. EpidemiologyAverage time of peak rotavirus activity in
16. EpidemiologyEstimated global distribution of the 800,000 annual deaths caused by rotavirus diarrhea.
17. Diagnosis and TreatmentAntigen Enzyme Immunoassay (EIA) of
18. Diagnosis and TreatmentA Doctor Examining a Dehydrated Child
19. Rotavirus vaccineMonovalent Vaccines - Live
20. Rotavirus vaccine Production of reassortant
21. PREVENTIONCDC program1) Hand washing2) Proper sanitation3)
22. Скачать презентанцию

MorphologyFamily Reoviridae 70-85 nm diameter Nearly spherical icosahedral particle Non-enveloped, double-shelled viruses Wheel-like distinct appearance under EM

Слайды и текст этой презентации

Слайд 1ROTAVIRUSES
by
WINDA ZULAIHA SHAHABUDIN
GROUP 24

Слайд 2

Слайд 3Morphology
Family Reoviridae

70-85 nm diameter

Nearly spherical icosahedral particle

Non-enveloped,

double-shelled viruses

Wheel-like distinct appearance under EM

MorphologyFamily Reoviridae 70-85 nm diameter Nearly spherical icosahedral particle Non-enveloped, double-shelled viruses Wheel-like distinct appearance under EM

Слайд 4Genome
11 segments of double-stranded RNA
structural viral proteins (VP):

outer/inner capsids: VP4 and VP7core: VP2, VP6, VP1, VP3

nonstructural proteins

(NSP): NSP1-5

Genome11 segments of double-stranded RNA structural viral proteins (VP): outer/inner capsids: VP4 and VP7core: VP2, VP6,

Слайд 5Genome

Слайд 6Classification
defined by cross-neutralization
with polyclonal antibodies of

antigenic
specificities (glycoproteins)

VP4 antigen: P serotype;

8 human rotaviruses

VP7 antigen: G serotype; 10 human rotaviruses

commonly found strains: P[8]G1; P[8]G3; P[8]G4;
and P[4]G2

Classificationdefined by cross-neutralization with polyclonal antibodies of antigenic specificities (glycoproteins) VP4 antigen:

Слайд 7Classification
Distribution of rotavirus strains from a global collection of 2,748

strains. "Others" includes strains that were not typable.

ClassificationDistribution of rotavirus strains from a global collection of 2,748 strains.

Слайд 8Replication
Attached to cell receptors contained sialic acid

Internalized and uncoated via

endolysosomes

Early transcription by viral RNA polymerase occurs inside sub-viral particle

Resulted in synthesis of (+) mRNAs and are translated in the cytoplasm.

Reassortment occurs during Early transcription.

ReplicationAttached to cell receptors contained sialic acidInternalized and uncoated via endolysosomesEarly transcription by viral RNA polymerase occurs

Слайд 9Replication
Secondary transcription occurs in cytoplasm in later

infection in a conservative fashion.

Uncapped non-polyadenylated transcripts

Particles assemble in

the cytoplasm 6-7 h after infection

Budding from the E.R. into internal spaces & are
eventually released when the cell lyses.

ReplicationSecondary transcription occurs in cytoplasm in later infection in a conservative fashion. Uncapped non-polyadenylated transcriptsParticles

Слайд 10Replication

Слайд 11Pathogenesis
infect upper two-third of duodenal epithelial cell

infectious particles are

released to intestinal lumen
and undergo further replication

in distal areas

cause severe diarrhea, vomiting and abdominal pain
among children

2 days incubation with 3-8 days watery diarrhea

death of over 600,000 children annually worldwide

Pathogenesisinfect upper two-third of duodenal epithelial cell infectious particles are released to intestinal lumen and

Слайд 12Pathogenesis

Слайд 13Epidemiology
Primary transmission mode is
fecal-oral

ingestion of contaminated

food or water and contact with contaminated surfaces

Annual epidemics occurring

from November to April

high rates of illness among infants and children below 2 years old, but mild among adults

EpidemiologyPrimary transmission mode is fecal-oralingestion of contaminated food or water and contact with contaminated surfacesAnnual

Слайд 14Epidemiology
Rotavirus causes the highest level of diarrhea among developed and

developing countries

Слайд 15Epidemiology
Average time of peak rotavirus activity in the contiguous 48

states,
United States, July 1991 to June 1997.

EpidemiologyAverage time of peak rotavirus activity in the contiguous 48 states, United States, July 1991 to June

Слайд 16Epidemiology
Estimated global distribution of the 800,000 annual deaths caused by

rotavirus diarrhea.

Слайд 17Diagnosis and Treatment
Antigen Enzyme Immunoassay (EIA) of stool specimens

RT-PCR;

not commonly done

Non-specific treatment: oral rehydration therapy to

prevent dehydration

Intravenous fluid is required in severe infant cases

Immunization by vaccines

Diagnosis and TreatmentAntigen Enzyme Immunoassay (EIA) of stool specimens RT-PCR; not commonly done Non-specific treatment: oral rehydration

Слайд 18Diagnosis and Treatment
A Doctor Examining a Dehydrated Child

Слайд 19Rotavirus vaccine
Monovalent Vaccines
- Live attenuated vaccine derived

from nonhuman host rotaviruses such as bovine and rhesus

Reassortant

Vaccines
- animal-human reassortants
expressing VP7 proteins used as immunogens such
as rhesus-human reassortant or
bovine-human reassortant vaccines

Rotavirus vaccineMonovalent Vaccines - Live attenuated vaccine derived from nonhuman host rotaviruses such as bovine

Слайд 20Rotavirus vaccine
Production of reassortant
tetravalent vaccine with

VP7 serotype 1-4 specificity

co-infection of RRV with HRV serotype

1,2, and 3

safe and immunogenic

Rotavirus vaccine Production of reassortant tetravalent vaccine with VP7 serotype 1-4 specificity co-infection of RRV

Слайд 21 PREVENTION
CDC program
1) Hand washing

2) Proper sanitation

3) Safe drinking water and

food

“Boil it, cook it, peel it, or forget it”

PREVENTIONCDC program1) Hand washing2) Proper sanitation3) Safe drinking water and food“Boil it, cook it, peel it,

Скачать презентацию

Разделы презентаций

Rotaviruses

Содержание

Слайды и текст этой презентации

Слайд 1ROTAVIRUSESbyWINDA ZULAIHA SHAHABUDINGROUP 24

Слайд 2

Слайд 3MorphologyFamily Reoviridae 70-85 nm diameter Nearly spherical icosahedral particle Non-enveloped,

double-shelled viruses Wheel-like distinct appearance under EM

Слайд 4Genome11 segments of double-stranded RNA structural viral proteins (VP):

outer/inner capsids: VP4 and VP7core: VP2, VP6, VP1, VP3nonstructural proteins

Слайд 5Genome

Слайд 6Classificationdefined by cross-neutralization with polyclonal antibodies of

antigenic specificities (glycoproteins) VP4 antigen: P serotype;

Слайд 7ClassificationDistribution of rotavirus strains from a global collection of 2,748

strains. "Others" includes strains that were not typable.

Слайд 8ReplicationAttached to cell receptors contained sialic acidInternalized and uncoated via

endolysosomesEarly transcription by viral RNA polymerase occurs inside sub-viral particle

Слайд 9ReplicationSecondary transcription occurs in cytoplasm in later

infection in a conservative fashion. Uncapped non-polyadenylated transcriptsParticles assemble in

Слайд 10Replication

Слайд 11Pathogenesisinfect upper two-third of duodenal epithelial cell infectious particles are

released to intestinal lumen and undergo further replication

Слайд 12Pathogenesis

Слайд 13EpidemiologyPrimary transmission mode is fecal-oralingestion of contaminated

food or water and contact with contaminated surfacesAnnual epidemics occurring

Слайд 14EpidemiologyRotavirus causes the highest level of diarrhea among developed and

developing countries

Слайд 15EpidemiologyAverage time of peak rotavirus activity in the contiguous 48

states, United States, July 1991 to June 1997.

Слайд 16EpidemiologyEstimated global distribution of the 800,000 annual deaths caused by