Polysaccharides: Quantitative analysis

by spectrophotometry
6. Drawing the curve (density/concentration)
7. Calculation of polysaccharides amount

Method: Dreywood’s method of spectrophotometric identification

flask
Water bath for 1 hour
Cooling, filtration

ethanol
-> mixed

2 ml
Cooling, centrifugation for 10 min, 3000 rpm

rpm
Drying of precipitate
Super is outlet

solution if poured into the 25 ml flask and the

Water bath, 10 min

in 10mm cuvets
Wavelength – 430nm
Reference – 4ml of reagent (antron

of density with known concentration of xylose. All conditions and

xylose and absolutely dry yield is calculated by following formula.

Where, c- concentration of xylose found in calibration curve (mkg/ml); kV – diffusion coefficient (2500); 0,91 – hydrolysis coefficient; 106 –coefficient of recalculation of mg into mkg; m – weight of initial seeds (g); W –loos of weight due drying of sample, %.

of polysaccharides of plant nature. The advantage is the availability