Разделы презентаций
- Разное
- Английский язык
- Астрономия
- Алгебра
- Биология
- География
- Геометрия
- Детские презентации
- Информатика
- История
- Литература
- Математика
- Медицина
- Менеджмент
- Музыка
- МХК
- Немецкий язык
- ОБЖ
- Обществознание
- Окружающий мир
- Педагогика
- Русский язык
- Технология
- Физика
- Философия
- Химия
- Шаблоны, картинки для презентаций
- Экология
- Экономика
- Юриспруденция
Experimental animal research in Almazov Centre Michael Galagudza 25.02.16,
Содержание
- 1. Experimental animal research in Almazov Centre Michael Galagudza 25.02.16,
- 2. Division of Experimental Physiology and Pharmacology –
- 3. Январь 2013 г.Renovation of the building of Institute of Experimental MedicineDecember 2012May 2013December 2013June 2014
- 4. Maintenance of 2000 mice and 1000 rats
- 5. ABSL-1 biosafety levelIndividually ventilated cagesCleanrooms of 8th
- 6. How do we work ?Surgery roomAnimal holding room
- 7. Aseptic surgical models in chronic experimentsComplete characterization
- 8. Experience and expertiseEquipment and techniquesPrerequisitesSurgical models +
- 9. Targeted drug delivery to the ischemic muscle
- 10. Heart-targeted delivery of cardioprotective drugs: focusing pharmacological
- 11. Potentiation of infarct-limiting effect of adenosine after
- 12. Linkermann, Green. N Engl J Med 2014; 370:455-465 Necrosome activators as therapeutic target
- 13. Cardioprotective effects of necroptosis inhibitors necrostatin-1 and
- 14. Cardioprotective effects of necroptosis inhibitor necrostatin-7 in
- 15. Reduced form: NADH Oxidized form: NAD+ 2402803203600481216Wavelength,
- 16. The dynamics of autofluorescence in repeated episodes
- 17. Intravital and post-mortem visualization of ischemic-reperfused area
- 18. Endovascular occlusion of middle cerebral arteryIschemic area
- 19. Shmonin et al. Int J Stroke. 2014;
- 20. Zukhurova et al., Phytother Res 2013; 27(9):
- 21. Mice – 17 000 Rats – 5
- 22. Welcome to the Institute of Experimental Medicine!
- 23. Скачать презентанцию
Division of Experimental Physiology and Pharmacology – 10 employees; Animal house – 6 employees;Laboratory of Myocardial Metabolism – 8 employees;Laboratory of Microcirculation – 8 employees;Laboratory of Mathematical Modeling – 8 employees;Laboratory
Слайды и текст этой презентации
Слайд 1Experimental animal research in Almazov Centre
Michael Galagudza
25.02.16, Almazov Centre,
St-Petersburg
Слайд 2Division of Experimental Physiology and Pharmacology – 10 employees;
Animal
house – 6 employees;
Laboratory of Myocardial Metabolism – 8 employees;
Laboratory
of Microcirculation – 8 employees;Laboratory of Mathematical Modeling – 8 employees;
Laboratory of Nanotechnology – 6 employees;
Laboratory of Systemic Hemodynamics – 6 employees;
Laboratory of Bioprosthetics and Cardioprotection – 6 employees.
TOTALLY 58 employees
Structure of the Institute of Experimental Medicine
Слайд 3Январь 2013 г.
Renovation of the building of Institute of Experimental
Medicine
December 2012
May 2013
December 2013
June 2014
Слайд 4Maintenance of 2000 mice and 1000 rats in the individually
ventilated cages
Maintenance of immune deficient animals and genetically engineered animals
(transgenic, knockout, …)New building of the Institute: animal house
Слайд 5ABSL-1 biosafety level
Individually ventilated cages
Cleanrooms of 8th and 6th classes
according to ISO definition
Barrier system (showers, pressure gradients, sticky pads,
etc.Backing up all main technological processes
The SPF animals are received from certified breeders only
External health monitoring program
Sterilization of all materials coming to the clean area
Regular education/training of the personnel
The technological principles of SPF barrier facility
Слайд 7Aseptic surgical models in chronic experiments
Complete characterization of cardiovascular system:
-
Echo
MRI
LDF
- cardiac catheterization (PV loops)
- isolated heart, in
vitro vessel reactivityAssessment of acute and chronic toxicity
Investigation of specific activity of the drugs
Specific forms of toxicity
New building of the Institute: novel opportunities
Слайд 8Experience and expertise
Equipment and techniques
Prerequisites
Surgical models + physiological monitoring, ECG,
Echo, MRI, PV loops, isolated heart, …
Outcomes
Implementation
Modular principle in planning
and designing research projects
Слайд 9Targeted drug delivery to the ischemic muscle with nanocarriers (particularly,
magnetic field-guided delivery);
Pharmacological and non-pharmacological neuroprotection: harnessing the potential of
cerebral pre- and postconditioning;Stimulation of myocardial regeneration with mesenchymal stem cells and apoptotic bodies;
Pharmacological cardioprotection using necroptosis inhibitors
(Auto)fluorescent imaging of ischemia-reperfusion injury
Main current research projects
Слайд 10Heart-targeted delivery of cardioprotective drugs: focusing pharmacological postconditioning on the
injury
Multifunctional nano-carrier
Target cell
Intact cell
Expression of injury marker
Слайд 11Potentiation of infarct-limiting effect of adenosine after its adsorption on
the surface of silica nanoparticles
** - р
- р<0,05 vs AdoI.v. infusion of ADO or ADO+SNPs (10 min)
Infarct size
30-min ischemia
Reperfusion 90 min
Galagudza et al., Int J Nanomed 2012; 7:1671–78
Слайд 12Linkermann, Green. N Engl J Med 2014; 370:455-465
Necrosome activators
as therapeutic target
Слайд 13Cardioprotective effects of necroptosis inhibitors necrostatin-1 and -5
Dmitriev et al.,
Eur Heart J, 2013; 34: 1021 (abstract)
Слайд 14Cardioprotective effects of necroptosis inhibitor necrostatin-7 in the in vivo
model
3 weeks
Dmitriev et al., Circulation 2014; 130: A17348 (abstract)
Слайд 15Reduced form: NADH
Oxidized form: NAD+
240
280
320
360
0
4
8
12
16
Wavelength, nм
Absorbance
NADH
NAD+
Ischemia
Ischemia-induced imbalance between
NAD and NADH can influence the level of autofluorescence
Слайд 16The dynamics of autofluorescence in repeated episodes of myocardial ischemia
Visualization
of early signs of myocardial injury in ischemia-reperfusion using NADH
autofluorescence
Слайд 17Intravital and post-mortem visualization of ischemic-reperfused area of the heart
with cardiogreen and TTC
Sonin et al., 2016 (in preparation)
Слайд 18Endovascular occlusion of middle cerebral artery
Ischemic area
(Evans blue)
Necrotic area
(TTC staining)
Silicone-processed prolene (4-0) filament
Filament position
Слайд 19Shmonin et al. Int J Stroke. 2014; 9:793-801.
The characteristics of
cerebral infarction in five different models of MCA ligation through
craniectomy
Слайд 20Zukhurova et al., Phytother Res 2013; 27(9): 1282-7
L-theanine administration results
in neuroprotection and prevents glutamate receptor agonist-mediated injury
Слайд 21Mice – 17 000
Rats – 5 000
Frogs (Xenopus
laevis) – 250
Fish (Danio rerio) – 34 000
Mini-pigs – 10
Monkeys
Molecular
imaging Cell biology/cytotoxicology
Molecular biology and genetics
Toxicology and carcinogenesis
Biochemistry
Morphology
Pharmacology and Physiology
Chemistry and Analysis
Embryogenesis
Immunotoxicology
Microbiology
Transgenic core facility
Divisions:
Animals:
New preclinical research center – 10 000 m2
