Preparative electrophoresis

Содержание

1. Preparative electrophoresis
2. Electrophoresis by the purpose of application is
3. Preparative electrophoresis Stages Separation Elution Polyacrylamide gel
4. Separation -+sampleCut ofElution
5. The Model 422 electro-eluter is an electroelution
6. Molecules are retained by dialysis membrane which
7. *Note: After elution volatile buffer is lyophilized in a spin-vacuum, leaving concentrated protein
8. Thanks for attention
9. Скачать презентанцию

Electrophoresis by the purpose of application is divided to analytical and preparative electrophoresis. Analytical electrophoresis- for the determination of the sample composition and rarely for the obtaining little amount of separating

Слайды и текст этой презентации

Слайд 1Preparative electrophoresis
Done by: Naizabayeva D.
Accepted by: Kenzhebayeva S.S.

Слайд 2Electrophoresis by the purpose of application is divided to analytical

and preparative electrophoresis.
Analytical electrophoresis- for the determination of the

sample composition and rarely for the obtaining little amount of separating substance (protein )

Preparative electrophoresis– targets the obtaining significant amount of purely separated substance (protein)

Electrophoresis by the purpose of application is divided to analytical and preparative electrophoresis. Analytical electrophoresis- for the

Слайд 3Preparative electrophoresis
Stages
Separation
Elution
Polyacrylamide gel electrophoresis
Gel preparation
Sample

adding
Separation
Staining
Cutting of certain segment (band) of separated protein
Elution by

the electrophoresis

Column electrophoresis

Preparative electrophoresis Stages Separation Elution Polyacrylamide gel electrophoresis Gel preparationSample addingSeparation StainingCutting of certain segment (band) of

Слайд 4Separation
-
+
sample
Cut of
Elution

Слайд 5 The Model 422 electro-eluter is an electroelution cell for preparative

recovery of biomolecules from agarose and acrylamide gels. The eluter

combines with the tank and lid of the Mini Trans-Blot® cell (or older Mini-PROTEAN® II or Mini-PROTEAN 3 cells) to elute macromolecules from single or multiple gel slices. Easy to assemble, the electro-eluter has six vertical glass tubes connecting the upper and lower buffer chambers.

Depending on the buffer system, the Model 422 electro-eluter can be used for elution or dialysis. Setup is quick and easy and the sample is collected in 400–600 µl. The Model 422 electro-eluter can be used for one to six samples without increasing the run time (3–5 hr) or decreasing sample yield.

A frit at the bottom of each tube retains the gel slice but permits macromolecules to migrate through when current is applied. When the macromolecules have passed through the frit, they are collected in the membrane cap for further analysis or testing.

The Model 422 electro-eluter is an electroelution cell for preparative recovery of biomolecules from agarose and acrylamide

Слайд 6Molecules are retained by dialysis membrane which is molded into

a cap
Membrane cap may be reused for at least five

complete runs without decreasing the yield. Refrigerate the membrane cap in elution buffer with 0,05% sodium azide (NaN3). It is not necessary to reheat or resoak the membrane caps after the first use.

Molecules are retained by dialysis membrane which is molded into a capMembrane cap may be reused for

Слайд 7*Note: After elution volatile buffer is lyophilized in a spin-vacuum,

leaving concentrated protein

Слайд 8Thanks
for
attention

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Preparative electrophoresis

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Слайды и текст этой презентации

Слайд 1Preparative electrophoresis
Done by: Naizabayeva D.
Accepted by: Kenzhebayeva S.S.

Слайд 2Electrophoresis by the purpose of application is divided to analytical

and preparative electrophoresis.
Analytical electrophoresis- for the determination of the

Слайд 3Preparative electrophoresis
Stages
Separation
Elution
Polyacrylamide gel electrophoresis
Gel preparation
Sample

adding
Separation
Staining
Cutting of certain segment (band) of separated protein
Elution by

Слайд 4Separation
-
+
sample
Cut of
Elution

Слайд 5 The Model 422 electro-eluter is an electroelution cell for preparative

recovery of biomolecules from agarose and acrylamide gels. The eluter

Слайд 6Molecules are retained by dialysis membrane which is molded into

a cap
Membrane cap may be reused for at least five

Слайд 7*Note: After elution volatile buffer is lyophilized in a spin-vacuum,

leaving concentrated protein

Слайд 8Thanks
for
attention

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Preparative electrophoresis

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Слайд 1Preparative electrophoresisDone by: Naizabayeva D.Accepted by: Kenzhebayeva S.S.

Слайд 2Electrophoresis by the purpose of application is divided to analytical

and preparative electrophoresis. Analytical electrophoresis- for the determination of the

Слайд 3Preparative electrophoresis Stages Separation Elution Polyacrylamide gel electrophoresis Gel preparationSample

addingSeparation StainingCutting of certain segment (band) of separated proteinElution by

Слайд 4Separation -+sampleCut ofElution

Слайд 5 The Model 422 electro-eluter is an electroelution cell for preparative

recovery of biomolecules from agarose and acrylamide gels. The eluter

Слайд 6Molecules are retained by dialysis membrane which is molded into

a capMembrane cap may be reused for at least five

Слайд 7*Note: After elution volatile buffer is lyophilized in a spin-vacuum,

leaving concentrated protein

Слайд 8Thanks for attention

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Что такое TheSlide.ru?

Слайд 1Preparative electrophoresis
Done by: Naizabayeva D.
Accepted by: Kenzhebayeva S.S.

and preparative electrophoresis.
Analytical electrophoresis- for the determination of the

Слайд 3Preparative electrophoresis
Stages
Separation
Elution
Polyacrylamide gel electrophoresis
Gel preparation
Sample

adding
Separation
Staining
Cutting of certain segment (band) of separated protein
Elution by

Слайд 4Separation
-
+
sample
Cut of
Elution

a cap
Membrane cap may be reused for at least five

Слайд 8Thanks
for
attention