recovery of biomolecules from agarose and acrylamide gels. The eluter
combines with the tank and lid of the Mini Trans-Blot® cell (or older Mini-PROTEAN® II or Mini-PROTEAN 3 cells) to elute macromolecules from single or multiple gel slices. Easy to assemble, the electro-eluter has six vertical glass tubes connecting the upper and lower buffer chambers.
Depending on the buffer system, the Model 422 electro-eluter can be used for elution or dialysis. Setup is quick and easy and the sample is collected in 400–600 µl. The Model 422 electro-eluter can be used for one to six samples without increasing the run time (3–5 hr) or decreasing sample yield.
A frit at the bottom of each tube retains the gel slice but permits macromolecules to migrate through when current is applied. When the macromolecules have passed through the frit, they are collected in the membrane cap for further analysis or testing.