Kazakh National Medical University named after S.D. Asfendiayrov   Department

Microbiology, Virology and Immunology  
The specialty: Stomatology
Lecture 2: Infection. Forms of

infection. Immunity. Types and forms of umminity. Factors specific and nonspecific defense. Serological reaction. Antigens. Antibodies. The Basic principles of immunization and immunotherapy

pathological processes, emerging and developing in the body when introducing

him pathogens that cause a violation of the constancy of its internal environment and physiological responses (Timakov). - invasion of a host organism by microorganism, proliferation of the invading organism, and host reaction

For the development of the infectious process must be 3 factors:
The pathogenic microbe
The susceptible microorganism
Certain environmental conditions

distinguished from other diseases::
The presence of a pathogenic microbe

The contagiousness
Cyclicality (proceeds periods)
Specific reactions of the organism to the pathogen
Development of immunity
Bacteria carriage

(species characteristic). VIRULENCE of microbes - is the degree of pathogenicity

(the strain sign).

Pathogenicity factors of microbes:
Adhesion
COLONIZATION – presence of microorganisms on skin or mucosa, no penetration into tissues.
Invasions - the penetration and proliferation associated with the introduction of live tissue (due to the enzyme hyaluronidase, neuraminidase, plasma coagulase)
Suppression of phagocytosis (by capsule, M protein from streptococcal protein A from Staphylococcus, cord factor in the tubercle bacillus)
AGRESSINS - substances that suppress the body's defenses and enhancing pathogens
Toxin - a poisonous substance produced by pathogenic microbes. Divided into exo-and endotoxins.

are highly toxic. Characterized Organotropona, virulence, antigenicity, immunogenicity. By the

mechanism of action are divided into : - Neurotoxins (tetanus) - Histo toxins (diphtheria) - Enterotoxins (cholera) - Hemolysin (lysis of red blood cells - strep) -leycocidins (staph) Can be transformed into anatoxin - exotoxin is deprived of toxicity, but has antigenic and immunogenic properties. It is used to prevent infections. Endotoxins - thermostable lipopolysaccharide (LPS), a part of the cell wall, gram (-) are detection of the destruction of bacteria. They do not have specificity. The antigenicity and immunogenicity is the weak. The cause of cardiac depression and low body temperature.

- dosis letalis minima - min. mortality. dose - the

smallest number of living microbes, causing the death of 80% -95% of the animals
Dlc - dosis letalis certa - certainly lethal dose - from which killed 100% of infected animals.
LD50 - dose of dies which 50% of infected animals
  DI - dosis infectionis - infective dose (the minimum number of microbial cells that can cause infectious process).

dose of pathogens (the minimum number of microbial cells capable

of causing infectious process)
Portal of entry - the body's tissues through which the organism enters the macro-organism.

of the disease (not contagious)
Prodromal - nonspecific common manifestations

(can be dangerous)
Height - the period of the development of clinical symptoms
Outcome:
recovery
death
bacteriocarrier

BY PRESENT CLINICAL SYMPTOMS
typical
atypical
BY LOCATION
total (generalized)
local

(alopecia)

within the cells, such as macrophages)
bacteriocarrier
DEGREE IN CLINICAL

EXPRESSION:
symptomatic (symptomatic)
abortive
latent
BY DESCENT:
exogenous
endogenous
autoinfection

animals) - brucelosis
anthropozoonoses (of people and animals) – plague
Sapronoses (dead

matter) - Legionella pneumophila

BY INTENSITY DISTRIBUTION:
Sporadic – isolated occurrence with no apparent connections between localities or times of occurrence
Group - a small number of cases in one community
Epidemic – significantly increased occurrence within a given localities and time periods
Pandemic - significantly increased occurrence within a given localities and time periods without restriction
The number of species of agent:
monoinfection (1 microbe)
mixed infections (mixed) - tank + virus

circulating in the blood
viremia - the virus circulates in

the blood
toxinemia (exotokisn) and toxemia (endo)
septicemia - microbes multiply in the blood
pyosepticemia - microbes multiply in the blood, are carried to the organs and tissues, there form secondary purulent foci.
sepsis (proliferation of microbes in the blood)
Reccurent infection:
secondary - to the existing inf-ii + new Notices
reinfection - sick with the same disease after complete recovery
superinfection - the patient during the illness are infected by the same pathogen
relapse - a return wedge. manifestations due to microbial residues after the first infection

permeability of the mucous membranes and conjunctive tissues
Neuraminidase –penetrates inside

the cell are distributed in the intercellular space.
Coagulase (thicken blood plasma)
Plasmin (dissolves fibrin clots)
Leukocidin (destroys white blood cells)
Lecithinase destroys cell membranes

infectious its NC - "infectivity"
The high specificity, Organotropona (there are

neurotropic viruses, hepatotropic viruses)
Blood viruses - transport environment, the presence of viremia stage.
Interaction of the viral genome and the genome of the cell
Infectious viruses - self-reproduce its genotype
Integration viruses - viral genes integrated into the chromosome of the cell and cause degeneration of cells (oncoviruses)
virus in immune system cells (lymphocytes) -virus influenza, measles, herpes, polio, AIDS, etc. Lymphotropic reflected in the outcome of the pathogenesis and viral infections (immunodeficiency)
The formation of intranuclear and intracytoplasmic inclusions - smallpox, rabies, herpes, measles, etc. Have diagnostic value.

of the virus in the cell and their rapid release:
focal
generalized
persistent
The

latent (asymptomatic) - the lack of virus isolation
Chronic -vydelenie virus from the body
Abortive - suspension of production
The development of neoplastic degeneration of cells (oncogenic viral infection)

resist harmful microorganisms or viruses from entering it.

The immune system

produces antibodies or cells that can deactivate pathogens.

Fungi, protozoans, bacteria, and viruses are all potential pathogens.

immunity germs are eliminated from the body) and non-sterile (produced

in the presence of germs)
a) an antimicrobial
b) antitoxic
c) antiviral
g) Antifungal
Natural passive (placental)
Artificial active (post-vaccination) - formed in a few weeks and lasts for several years
Artificial passive (postserum) - formed after a few hours and lasts for several weeks or months

cells destroy antigens by direct cytotoxicity and by the synthesis

of lymphokines.
The regulation of the immune response involves two subtypes of T cells: T helper enhance the immune response of T-suppressors have the opposite effect.

helper B

LM clone antibody-producing cells (plasma cells) immunoglobulins (antibodies) (Ig)
                 AH AT complex.

antigen. His expression reaches a maximum of 7 - 8

th day, persists for 2 weeks, and then decreases; (Ig M)
secondary immune response occurs at the second meeting with the antigen by the cells of immunological memory. The secondary immune response is developing rapidly due to the memory cells and reaches more (3 - 4 times) intensity;(Ig G)

:


1st, nonproductive - antigen recognition and interaction of immune cells;

2nd,

productivity - the proliferation of effector cells and antibody production.

against pathogens uses mostly physical and chemical barriers such as

Skin – acts as a barrier to invasion
Sweat – has chemicals which can kill different pathogens.
Tears - have lysozyme which has powerful digestive abilities that render antigens harmless.
Saliva – also has lysozyme.
Mucus - can trap pathogens, which are then sneezed, coughed, washed away, or destroyed by chemicals.
Stomach Acid – destroys pathogens

in increased blood flow

Heat - due to capillary dilation

resulting in increased blood flow

Swelling – due to passage of plasma from the blood stream into the damaged tissue

Pain – due mainly to tissue destruction and, to a lesser extent, swelling.

promotes the maturation of the immune system

(natural killer cells)
  lymfocit-shared population of cells possessing the natural

cytotoxicity
Antiviral
Antitumor
Antiprotozoal

tissue macrophages. The marked effect on the Gram+ bacteria

The complement

system -20 regulatory serum proteins
Pathway:
1. Classic Ag+ AT C1, C4, C2 C3

2. Alternative LPS properdin, Mg 2+ C3

Cytokines - hormone-like mediators (interleukins, interferons, growth factors), produced by various cells of the body and can affect the function of other or the same group of cells

function as a single unit, protecting the body against infections

and foreign agents

Features of the immune system:
The cells are spread throughout the body
The cells are circulating in the blood
Constantly develops AT
It consists of 1012 lymphoid cells
The total weight of 1.5-2 kg
The central figure - lymphocyte

responses, which have receptors AG
Auxiliary - (antigen) - the ability

to distinguish foreign cells from their own and submit them to immunocompetent cells.
Cells AG-nonspecific defense that distinguish the body's own components from foreign particles and destroy them

immune response
T LM (80% lymphocytes)
T - effectors
T - killers
T -

helpers
T – suppressor

Features:
cell-mediated immunity;
regulation of the activity of B-cells (immunologic memory and tolerance);
hypersensitivity (IV) type.
graft rejection;
antitumor immunity

Participants in the antigen presentation of T lymphocytes

specific immune response and to participate in its implementation
Properties:
Alien
The antigenicity
Specificity
Immunogenicity
Protein

nature
High MR
Types AG
Full - capable of inducing the formation of specific antibodies and to react with them
Haptens - failed to induce the formation of specific antibodies and to react with them
The structure of the AG 2 components:
Protein - defines the antigenicity
The amino acid residues (determinant group) located on the surface of the protein - specificity.

белок

types of microorganisms, animals and plants. For example, Antigens Forsman

- guinea pig, e / c sheep and Salmonella.
Cross-react AG (PRA) - found in a number of micro-organisms and in human tissues. For example, hypertension hemolytic streptococcus, the human myocardium and renal glomerulus, so provokes rheumatic heart disease and glomerulonephritis.
Izoantigens - some of them individuals or groups of individuals differ (ABO blood)
Tumor - as a result of malignant transformation
Viral - linked to the nucleocapsid or envelope glycoproteins
HLA - Antigens major histocompatibility complex
Somatic - thermostable O Antigens
The flagellar - labile H Antigens
Capsule - labile K - Antigens
Antigens virulence - Vi - Antigens
Autoantigens (glass. Body, the thyroid gland)

AG and does not produce antibodies. Occurs when the body

met with antigens in the embryonic period, when the defects of the lymphoid tissue, when very high or very low doses of antigen in an organism with a weak Immunity system.
Immunological paralysis - the inability organism produce AT form when very high doses of antigen. Due to blockade of immunocompetent cells. After removing unnecessary AG products AT resumes.
Immunodeficiency - reduction or absence of humoral and cellular defense. congenital and acquired.

antigen.
Structure of Antibodies

L Н Н L
Fab-фрагмент V участок

s-s
s-s


Fc-фрагмент С участок

A)
Ig E
Ig D

height of the primary immune response and the immune response

again. It penetrates through the placenta to the fetus.
▪ Ig M (13%). The first start synthesized in the body of the fetus and the first to appear in the serum after immunization. Do not cross the placenta.
▪Ig A (40%) is synthesized by plasma cells in the spleen and lymph nodes. The average concentration of them - 2.5 g / l.
Ig D (75%) did not cross the placenta. They can play a role in the malignant transformation of cells.
Ig E (0,00025 g / tracks) synthesized by plasma cells and are involved in anaphylactic reactions (reagin).

specific immune response by mobilizing mechanisms of immunological memory

Immune serum

and immunoglobulins - preparations containing completespecific antibodies, the introduction of which in the organism leads to the immediate acquisition of passive humoral immune response.

of the actual antigen that causes the disease. The injection

is too weak to make you sick, but your B lymphocytes will recognize the antigen and react as if it were the "real thing". Thus, you produce MEMORY cells for long term immunity.

(no significant side reactions)
HARMLESSNESS
MINIMUM sensitizing effect

development
Bacterial
viral

virulence) microbes retained immunogenicity. These vaccines are characterized by high

efficiency, as cause in the body similar to the natural process of infection, but without clinical manifestations. When this vaccine strain may persist and multiply in the body. Typically, once introduced.
Benefits:
A single injection
Prolonged immunity
Disadvantages:
- In a weakened organism can cause infections

inactivate microbes are used:
1. Elevated Temperature (56-58 ° C)
2. The

chemicals (ethyl alcohol, formalin, acetone, phenol)
3. UFO
Benefits:
They do not cause infectious disease in a weakened body
Disadvantages:
repeated administration
Immunity non-durable

by various treatments, in particular by enzymes (pancreatin, trypsin). These

less reactogenic vaccine, a storage stable, more immunogenic. They are made of several kinds of microbes, i.e. they are integrated (associates). The advantage of them in a sharp reduction in the number of injections, while maintaining the amount of antigen administered. Usually they are administered singly.
In order to antigens not quickly absorbed into the body and provide long-lasting immunity, they added absorbent material (aluminum hydroxide adjuvant, phosphate, aluminum)

formalin solution. It contains many ballast substances. Currently uses purified

toxoids adsorbed to the adjuvant. This toxin loses its virulence, but retains the ability to induce the synthesis of antitoxic antibodies.
Diphtheria toxoid adsorbed purified
Staphylococcal toxoid
Tetanus toxoid adsorbed purified
DT - toxoid
Polianatoksin peeled
Anatoxins connected with corpuscular AG (DTP cholera vaccine)

which creates artificial passive immunity acquired. Immunity is created quickly,

but lasts a short time, because introduced protein is rapidly degraded.
Sera have immediate effect, neutralizing toxins, destroying the bacteria themselves. Therefore, they are mainly used for the treatment and prophylaxis less.
Often introduced by intramuscular injection.

(derived from human blood)
heterologous:
Immunization of animals
The high concentration of antibodies
Unlimited

selection of producers
The high immunogenicity of the (alien) -
especially when using
homologous:
are not immunogenic
From the donor or from placental blood
AT concentration is not great. This may include other antibodies.

types:
Normal (obtained from the donor, abortive, placental blood)
measles
polio
pertussis
2. The direction

of action (immune sera obtained from human donors and animals)
Against rabies
smallpox
tetanus

production of diagnostic reactions.
Diagnosticums - a suspension of killed bacteria

of some kind. Their variety - erythrocyte diagnostic tools (bacterial AG adsorbed on erythrocytes).
Diagnostic serum - antibodies obtained by immunizing animals (rabbits, sheep) the relevant AG.
Allergens - preparations for the production of skin tests and detection of allergic conditions. Show infection of the body.