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Cellular Level of Organization

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Topics:RESEARCH METHODS IN CYTOLOGYCell. Membrane organellesCell. REMEMBRANCE ORGANELLESNucleus, chromosome

Слайды и текст этой презентации

Слайд 1Cellular Level of Organization
Lecture 2

Cellular Level of OrganizationLecture 2

Слайд 3Topics:
RESEARCH METHODS IN CYTOLOGY
Cell. Membrane organelles
Cell. REMEMBRANCE ORGANELLES
Nucleus, chromosome

Topics:RESEARCH METHODS IN CYTOLOGYCell. Membrane organellesCell. REMEMBRANCE ORGANELLESNucleus, chromosome

Слайд 4Topic: RESEARCH METHODS IN CYTOLOGY

Topic: RESEARCH METHODS  IN CYTOLOGY

Слайд 5RESEARCH METHODS IN CYTOLOGY
QUESTIONS
What studies Cytology.
The idea that organisms are

made up of cells.
Research methods used in Cytology.
Cell fractionation.
Autoradiography.
Determination of

the duration of some stages of the cell cycle by radio.

RESEARCH METHODS  IN CYTOLOGYQUESTIONSWhat studies Cytology.The idea that organisms are made up of cells.Research methods used

Слайд 6RESEARCH METHODS IN CYTOLOGY
What studies Cytology (I)
Cytology - the science

of the cell.
From among other biological Sciences, it stood

out almost 100 years ago.
For the first time generalized information about the structure of cells were collected in the book Zh.-B. Karnua «Сell Biology", published in 1884.
Modern Cytology studies:
structure of cells, their functioning as elementary living systems;
the functions of individual cell components;
the process of reproduction of cells and their reparations;
adaptation to environmental conditions and many other processes that allow to judge the properties and functions common to all cells.
Cytology also considers the features of the structure of specialized cells.
RESEARCH METHODS  IN CYTOLOGYWhat studies Cytology (I)Cytology - the science of the cell. From among other

Слайд 7RESEARCH METHODS IN CYTOLOGY
What studies Cytology (II)
Cytology is closely related

to
Biochemistry
Biophysics
Molecular biology
Genetics
The discipline "Cytology" refers to the fundamental sections of

biology, because it explores and describes the only unit of all life on Earth – the cell.
RESEARCH METHODS  IN CYTOLOGYWhat studies Cytology (II)Cytology is closely related toBiochemistryBiophysicsMolecular biologyGeneticsThe discipline

Слайд 8Cell theory
In the XVII century. Robert Hooke, a physicist and

biologist, characterized by great ingenuity, created a microscope. He called

small units cells.
in 1839, Matthias Schleiden (Germany) and Theodor Schwann (Germany) independently formulated the cell theory
Cell theoryIn the XVII century. Robert Hooke, a physicist and biologist, characterized by great ingenuity, created a

Слайд 9The main positions of the cell theory (I)
The cell is

the basis of structural and functional organization of plants and

animals;

Plant and animal cells are similar in structure and develop similarly (by dividing the original cell);

Cells in all organisms have a membrane structure;

The nucleus of a cell represents its main regulatory organoid;

Cellular structure of living organisms is the evidence of the unity of their origin
The main positions of the cell theory (I)The cell is the basis of structural and functional organization

Слайд 10The main positions of the cell theory (II)
Additions were made

to the cell theory in the period of 1855-1859

Rudolf

Virchow (Germany):

Cells arise only from cells through reproduction. This point of the cell theory excludes the possibility of cell formation from the non-cellular material;

The cell is a unit of pathology..
The main positions of the cell theory (II)Additions were made to the cell theory in the period

Слайд 12RESEARCH METHODS IN CYTOLOGY
Research methods in Cytology
Ordinary light microscopy, which

has not lost its role.
Polarizing microscopy.
Ultraviolet microscopy.
Fluorescence microscopy.
Phase-contrast microscopy.
Electron

microscopy
RESEARCH METHODS  IN CYTOLOGYResearch methods in CytologyOrdinary light microscopy, which has not lost its role.Polarizing microscopy.Ultraviolet

Слайд 13RESEARCH METHODS IN CYTOLOGY
Modern research methods
The most useful in modern

times were:
1) Electron microscopy;
2) Fractionation of cells by

which biochemists can identify a relatively pure fraction of cells contain certain organelles, and to study, thus separate they are interested in metabolic reactions;
3) radioautography, which made it possible to directly study the individual metabolic reactions occurring in organelles.
RESEARCH METHODS  IN CYTOLOGYModern research methodsThe most useful in modern times were: 1) Electron microscopy; 2)

Слайд 14RESEARCH METHODS IN CYTOLOGY
Fractionation method
The method allows:
to separate different

organelles of the cell in a relatively pure form;
determine the

chemical composition of organelles and enzymes contained in them;
on the basis of the data obtained to draw conclusions about the functions of organelles in the cell.
to determine the chemical composition and enzymatic activity of the selected organelles.

RESEARCH METHODS  IN CYTOLOGYFractionation methodThe method allows: to separate different organelles of the cell in a

Слайд 15The stages of fractionation of the cell
Cell fractionation by centrifugation
Repeated

centrifugation at progressively higher speeds will fractionate homogenates of cells

into their components. In general, the smaller the subcellular component, the greater is the centrifugal force required to sediment it.
Typical values for the various centrifugation steps referred to in the figure are:
The stages of fractionation of the cellCell fractionation by centrifugationRepeated centrifugation at progressively higher speeds will fractionate

Слайд 16RESEARCH METHODS IN CYTOLOGY
Literature list
Molecular Biology of the Cell. 4th

edition.
Alberts B, Johnson A, Lewis J, et al. New York: Garland

Science; 2002
RESEARCH METHODS  IN CYTOLOGYLiterature listMolecular Biology of the Cell. 4th edition.Alberts B, Johnson A, Lewis J,

Слайд 17Topic: Cell. Membrane organelles

Topic: Cell. Membrane organelles

Слайд 18Questions:
General outline of the cell structure
Features of the structure of

prokaryotic cells
Comparison of plant and animal cells
Characteristic of

membranes
Lysosomes
Golgi apparatus
Endoplasmic reticulum
Mitochondria
Plastids
Vacuole
Metabolic cycle in a cell
Questions:General outline of the cell structureFeatures of the structure of prokaryotic cells Comparison of plant and animal

Слайд 19General outline of the cell structure

General outline of the cell structure

Слайд 21SHELL
(cell wall + cytoplasmic membrane in plants
or cytoplasmic membrane

in animals)
PROTOPLASM
(the contents of the cell)
KARYOPLASM
(nucleus substance)
CYTOPLASM
(all other contents of

the cell)

CYTOSOL
(soluble part of cytoplasm)

ORGANOIDS
(structurally and functionally isolated parts of the cytoplasm)

SPARE NUTRIENTS
(organic and inorganic)

Cell

MEMBRANOUS
Lysosomes, Golgi apparatus, endoplasmic reticulum, mitochondria, plastids

Non-MEMBRANE
Cell center, microtubules, microfilaments, cilia, flagella

SHELL(cell wall + cytoplasmic membrane in plants or cytoplasmic membrane in animals)PROTOPLASM(the contents of the cell)KARYOPLASM(nucleus substance)CYTOPLASM(all

Слайд 22Features of the prokaryotic structure
Capsule (pectin mucosa)
Cell wall
Cytoplasmic membrane (CPM)
Nucleoid
Plasmids
Mesosome
Ribosomes

(size -
Fimbriae (pili)
Flagellum
Cytoplasm
(ring DNA, membrane of the cytoplasm is not

separated)

(contains murein, not cellulose)

Photosynthetic membrane

(invagination inside the CPM cells, a functional analog of the chloroplast)

(invagination inside the CPM cells, a functional analog of the mitochondrial)

(flagellin's protein thread moves due to the rotation of the inner ring, not the contraction of the thread itself)

(70 S, but not 80 S)

(contain additional genetic material)

(attachment to substrate and absorption of nutrients)

Features of the prokaryotic structureCapsule (pectin mucosa)Cell wallCytoplasmic membrane (CPM)NucleoidPlasmidsMesosomeRibosomes (size -Fimbriae (pili)FlagellumCytoplasm(ring DNA, membrane of the

Слайд 23Comparison of plant and animal cells:
TYPE OF NUTRITION
AUTOTROPHIC
(synthesis of organic

substances from inorganic)
HETEROTROPHIC
(synthesis of organic substances only from organic
Plant cell
Animal

cell

Cell wall

PLASTIDS
(the organelles responsible for autotrophic type of nutrition)

VACUOLE

in lower animals performs a digestive function

ATP synthesis

mitochondria, chloroplasts

mitochondria

CELLULAR CENTRE

only in lower animals

SPARE CARBOHYDRATES

starch

glycogen

Comparison of plant and animal cells:TYPE OF NUTRITIONAUTOTROPHIC(synthesis of organic substances from inorganic)HETEROTROPHIC(synthesis of organic substances only

Слайд 25Biological membrane
Protein molecules (80-20%) immersed in lipid bilayer (20-80%)
GLYCOCALYX
PERIPHERAL PROTEINS
INTEGRAL

PROTEINS
recognition of external signals adhesion of cells in the tissue
immune

response

form a hydrophilic channel - pore
transported through the membrane of certain substances (are carriers)

Biological membraneProtein molecules (80-20%) immersed in lipid bilayer (20-80%)GLYCOCALYXPERIPHERAL PROTEINSINTEGRAL PROTEINSrecognition of external signals adhesion of cells

Слайд 32Transport of substances through the plasma membrane
ACTIVE TRANSPORT
PASSIVE TRANSPORT
(DIFFUSION)
ENDOCYTOSIS
EXOCYTOSIS
PHAGOCYTOSIS
PINOCYTOSIS
TRANSFER
PENETRATION
Ordinary DIFFUSION
Facilitated

DIFFUSION
Binding of the dissolved substance
Carrier inversion
Release of dissolved substance
PERMEASE
As well

as the active transport is carried out by using protein vectors
Transport of substances through the plasma membraneACTIVE TRANSPORTPASSIVE TRANSPORT(DIFFUSION)ENDOCYTOSISEXOCYTOSISPHAGOCYTOSISPINOCYTOSISTRANSFERPENETRATIONOrdinary DIFFUSIONFacilitated DIFFUSIONBinding of the dissolved substanceCarrier inversionRelease of

Слайд 34Topic: Membrane organelles

Topic: Membrane organelles

Слайд 41Hypotheses of increasing the number of mitochondria in a cell:
Mitochondria

are formed from precursors (promitochondria)

Mitochondria are formed from other membrane

organelles of the cell

The increase in the number of mitochondria in the cell occurs as a result of the division of the mitochondria
Hypotheses of increasing the number of mitochondria in a cell:Mitochondria are formed from precursors (promitochondria)Mitochondria are formed

Слайд 46Topic: CELL. REMEMBRANCE ORGANELLES

Topic: CELL.  REMEMBRANCE ORGANELLES

Слайд 47RESEARCH METHODS IN CYTOLOGY
Questions :
Cell wall
Microtubules
Microfilament
Cell center
Ribosomes

RESEARCH METHODS  IN CYTOLOGYQuestions :Cell wallMicrotubulesMicrofilamentCell centerRibosomes

Слайд 49Cell wall in bacteria

Cell wall in bacteria

Слайд 59Topic: Nucleus. CHROMOSOME

Topic: Nucleus. CHROMOSOME

Слайд 60Questions:
Structure and functions of the nuclear apparatus of the cell
Definition

of chromosome
Structure and function of chromosomes
Types of chromosomes
Chromosomes of

"tube brushes" type
Polytene chromosomes
B-chromosomes
Questions:Structure and functions of the nuclear apparatus of the cellDefinition of chromosomeStructure and function of chromosomesTypes of

Слайд 62Chromosome
The chromosome is a permanent component of the nucleus, characterized

by a special structure, personality, function and ability to reproduce

itself, which ensures the continuity and transmission of hereditary information from one generation of living organisms to another.

Its name was due to the ability to intensely colored basic dyes. The term "chromosome" was proposed in 1888 by Valldaura.
Chromosome The chromosome is a permanent component of the nucleus, characterized by a special structure, personality, function

Слайд 72Functions of chromosomes
1. Information - contains DNA, which contains qualitatively

different genes that make up the genome of the cell.

2.

Transcription – the reading of the information contained therein in the course of gene expression.

3. Structural and organizational - provides extremely accurate reproduction of chromosomes during replication and identity of child chromosomes diverging to the poles.

4. Segregation - provides distribution of chromosomes along the poles in different spores or gametes in meiosis.

5. Recombination - provides a significant part of the combinative variability - recombination of linked genes.
Functions of chromosomes1. Information - contains DNA, which contains qualitatively different genes that make up the genome

Слайд 73Karyotype
Karyotype - chromosomal complex species with its own characteristics:


the number and size of chromosomes;
their morphology;
the presence

of under a light microscope parts of the structure, constrictions, satellites,
shoulder ratio, alternation of EU-and heterochromatin.

The most important property of the karyotype is the presence of pairs of homologous chromosomes.
KaryotypeKaryotype -  chromosomal complex species with its own characteristics: the number and size of chromosomes; their

Слайд 81Polytene chromosomes
It was discovered in 1881 by Balbiani

Characteristics:

in terms of

volume, about 1000 times more somatic due to 9 –

10 consecutive replication;

the cell cycle in cells of this type consists of only 2 phases: synthesis of DNA and synthetic period;

polytene chromosomes are not able to enter into mitotic divisions.

VALUE: due to polyteny, the body mass increases much faster than due to mitotic divisions of diploid cells.
Polytene chromosomesIt was discovered in 1881 by BalbianiCharacteristics:in terms of volume, about 1000 times more somatic due

Слайд 84CHROMOSOME TYPE "OF TUBE BRUSHES»
Were opened In 1878 by W.

Fleming

Can be found in meiosis at the stage of prophase

I, in diplotene

Formed in the course of extremely long meiotic division.

Chromosomes of type "Tube brushes" – diplotene bivalent, consisting of two chromosomes, which intersect in the points a chiasm.
CHROMOSOME TYPE

Слайд 85The generally accepted parts
of modern cell theory include:

All known

living things are made up of one or more cells
All

living cells arise from pre-existing cells by division.
The cell is the fundamental unit of structure and function in all living organisms.
The activity of an organism depends on the total activity of independent cells.
Energy flow (metabolism and biochemistry) occurs within cells.
Cells contain DNA which is found specifically in the chromosome and RNA found in the cell nucleus and cytoplasm.
All cells are basically the same in chemical composition in organisms of similar species.
The generally accepted parts of modern cell theory include:All known living things are made up of one

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