Of St. Petersburg. health Committee. St. Petersburg state budget professional educational
institution " Medical College №3»Discipline: "Histology with histological technique»Topic: "preparation of histological preparations»
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The Government Of St. Petersburg. health Committee. St. Petersburg state budget
Содержание
- 1. The Government Of St. Petersburg. health Committee. St. Petersburg state budget
- 2. Taking materialTaken for subsequent histological examination for diagnostic purposes. The material then register.
- 3. FixationIt is necessary to prevent the processes
- 4. WashingGetting rid of the excess retainer and
- 5. DehydrationWiring seals tissue, which is necessary for
- 6. ImpregnationIs made to remove material from the alcohol, to further fill in paraffin and celloidin.
- 7. CastingIt is necessary for long-term archiving and
- 8. Block stickerBlocks are glued to the wooden
- 9. Obtaining slices on a microtomeThis is a
- 10. Staining of sections and closing in optically
- 11. Microscopy
- 12. Thanks for watching!
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Taking materialTaken for subsequent histological examination for diagnostic purposes. The material then register.
Слайды и текст этой презентации
Слайд 1Preparedstudent gr. L-31.:Dmitry Plamadyala, Sytov Alexey, Berezan Ellina
The Government
Слайд 2Taking material
Taken for subsequent histological examination for diagnostic purposes. The
material then register.
Слайд 3Fixation
It is necessary to prevent the processes of decomposition, preserving
the structures of life. Usually use 10% formalin solution.
Слайд 4Washing
Getting rid of the excess retainer and precipitation of the
fixing liquid. Is on the unit wiring histological carousel type.
Слайд 5Dehydration
Wiring seals tissue, which is necessary for obtaining slices (if
the fabric is too soft, when microcomedone it will "wrinkle"
forming folds, breaks, and other artifacts, making it unusable to the study).
Слайд 6Impregnation
Is made to remove material from the alcohol, to further
fill in paraffin and celloidin.
Слайд 7Casting
It is necessary for long-term archiving and obtaining slices on
the microtome. Use special shapes to shape the block.
Слайд 8Block sticker
Blocks are glued to the wooden blocks to obtain
sections on the sled microtome and further preservation in the
archive.
Слайд 9Obtaining slices on a microtome
This is a mechanical device for
obtaining histological sections. On the sled microtome, slices of paraffin
and celloidin blocks are prepared. Consists of: bed limiters, micrometer, objectattributes and knife sled.
Слайд 10Staining of sections and closing in optically transparent walls
For the
preparation of sections for painting, they remove the wax or
celloidin. If the cut is obtained on the freezing microtome, it is immediately placed in the dye. Then, in the optically transparent medium.
