G11.4 Microorganisms and Biotechnology Pa rt 1 2017-2018

the main types of nutrition in microorganisms
11.4.3.2 Describe the features

aseptic technique?
Why is it important to use when working

Heterotrophs –

donors
Energy from H2 NH3 NO2

hydrocarbons…
Nitrogen – organic: amino acids, peptides, proteins

Nutrients

Agar 2.0 min https://www.youtube.com/watch?v=YX_b02KYN9g
Making a Streak plate 3.0 min https://www.youtube.com/watch?v=0heifCiMbfY

growth.
What is happening to the culture at time
=5.5-10 hours?

What

colonies from liquid agar culture so they may be easily

0 % Absorbance
20 % Transmittance
80 % Absorbance

Used to measure ‘turbidity’
concentration of bacterial in solution

– dead bacteria
precipitate out of solution
Sterile Broth
Significant turbidity
-lots of bacteria
Slight

Dead bacteria

to oxygen”


1. Obligate anaerobic bacteria
2. Obligate aerobic bacteria
3. Facultative

Condition

Environment

37C mesophiles
Hot 60C thermophiles
95C

Environment

(aerobic)
Facultative aerobes – prefer oxygen, but do fine without oxygen
Aerotolerant

Environment

Metabolism

in Liquid Culture
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neutrophils
9 and above – alkalphiles
Environment

of yeast for observation.
Practical II Plate it on different

1- Nutrient closed petri dish
2- No nutrient closed petri dish
3- Glucose closed petri dish
4 – No glucose closed petri dish
5 – Nutrient open petri dish
6 - No nutrient open petri dish
72 hours in incubator or 72 hours covered in warm part of room.

nanhalophiles

NaCl required (15%) halophiles

Environment

-What is a permanent stain?
-How is a permanent stain

of tissue with chemical agent.
Dehydration & Clearing – removal of

of the extracellular matrix.

2. Eosin – Stains that differentiate between

to detect polysaccharides (e.g. glycogen, glycoproteins, glycolipids and mucins in

the fermenter

when the resources are limited.
2. Population seldom grows beyond the

Exponential or J-shaped

1. It occurs when the resources are abundant.
2. Population passes well beyond the carrying capacity of the ecosystem.
3. A stationary or steady phase is seldom achieved.
4. Population crashes ultimately due to mass mortality.
5. It has two phases, lag and log. 
6. It occurs in fewer organisms, e.g., Lemmings, algal bloom.

of your Microbiology metabolism worksheet answer the following:

“With respect

curves:
Directions: For each of the following scenarios circle whether the