3' exo + 5' exo 103 kD
DNA
polymerase IIIa 130 kD polymerase
e 27 kD 3'-exo
q 9 kD
d 39 kD
c 17 kD
y 15 kD
g 48 kD
b 41 kD processivity factor
t 71 kD
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Replication in bacteria
Содержание
- 1. Replication in bacteria
- 2. ReplicationChemistry of replication
- 3. ReplicationChemistry of replication
- 4. ReplicationChemistry of replication
- 5. ReplicationFidelity of replication
- 6. ReplicationEnzymology of replication
- 7. ReplicationEnzymology of replication Replicative DNA polymerasesDNA
- 8. ReplicationEnzymology of replication Reparative DNA polymerasesDNA
- 9. ReplicationReplication fork
- 10. ReplicationReplication fork
- 11. ReplicationHelicases
- 12. ReplicationHelicasesDnaB6Rep2openclosed
- 13. ReplicationHelicasesWorking cycle of Rep2Translocation Unwinding
- 14. ReplicationReplication form components SSB
- 15. ReplicationPrimase
- 16. ReplicationPrimase
- 17. ReplicationRemoval of primer
- 18. ReplicationLigation of Okazaki fragments
- 19. ReplicationMechanism of ligation
- 20. ReplicationMechanism of ligation
- 21. ReplicationMechanism of ligation
- 22. ReplicationDNA polymerase III
- 23. ReplicationProcessivity factor (clamp)Processivity factor, clamp,has pseudo 6-foldsymmetry.In
- 24. ReplicationDNA polymerase III
- 25. ReplicationClamp loading
- 26. ReplicationClamp loading
- 27. ReplicationChemistry of replication
- 28. ReplicationSupercoiling problem ahead of replication fork
- 29. ReplicationProblems of concatemers resolution
- 30. ReplicationProblems of concatemers resolution
- 31. ReplicationProblems of concatemers resolution
- 32. ReplicationSimilar supercoiling problems are relevant for transcription
- 33. ReplicationOrigin of replication
- 34. ReplicationInitiation of replication is regulated by methylation of dam sites in ori region
- 35. ReplicationInitiation of replication
- 36. ReplicationChemistry of replication
- 37. ReplicationStart of replication
- 38. ReplicationTermination of replication
- 39. ReplicationTermination of replication
- 40. ReplicationHow to separate sister genomes into daughter
- 41. ReplicationProblem of genome dimersFtsK – ATPase that helps to separate genomes to daughter cells
- 42. ReplicationProblem of genome dimersXerC/XerD – recombinase is attracted to dif sites by FtsK
- 43. ReplicationProblem of genome dimersXerC/XerD – recombinase
- 44. ReplicationSeptum localization and growth
- 45. ReplicationSeptum localization and growthMinC inhibits FtsZ ring polymerization MinD interacts with MinC, assisting its polar localization
- 46. ReplicationSeptum localization and growthMinC/D cap on the
- 47. ReplicationTiming of cell divisionCell division time could
- 48. ReplicationSeveral factors contribute to regulation of initiation of replication
- 49. Скачать презентанцию
ReplicationChemistry of replication
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Слайды и текст этой презентации
Слайд 7Replication
Enzymology of replication
Replicative DNA polymerases
DNA polymerase I +
3' exo + 5' exo 103 kD
polymerase IIIa 130 kD polymerase
e 27 kD 3'-exo
q 9 kD
d 39 kD
c 17 kD
y 15 kD
g 48 kD
b 41 kD processivity factor
t 71 kD
Слайд 8Replication
Enzymology of replication
Reparative DNA polymerases
DNA polymerase II +
3' exo
90 kDDNA polymerase IV 40 kD
DNA polymerase V
umuD’ 15 kD
umuC’ 48 kD
Слайд 23Replication
Processivity factor (clamp)
Processivity factor, clamp,
has pseudo 6-fold
symmetry.
In E. coli b-clamp
is a
dimer of 3-domain
proteins
Т4 phage and eukariotes
contain trimer of 2-domain
proteins
(PCNA)
Слайд 40Replication
How to separate sister genomes into daughter cells?
FtsK –
ATPase that helps to separate genomes to daughter cells
GGGNAGGG sites
are located in polar fashion ori to dif
FtsK interacts with these sites and is loaded onto DNA in direction of dif
Слайд 41Replication
Problem of genome dimers
FtsK – ATPase that helps to separate
genomes to daughter cells
Слайд 42Replication
Problem of genome dimers
XerC/XerD – recombinase is attracted to dif
sites by FtsK
Слайд 45Replication
Septum localization and growth
MinC inhibits FtsZ ring polymerization
MinD interacts with
MinC, assisting its polar localization
Слайд 46Replication
Septum localization and growth
MinC/D cap on the pole is disassembled
by MinE ring MinC/D monomers diffuse to another pole, where they
form new cap While old cap disassembles, new one is growing After complete disassembly of the old cap, MinE ring forms on the edge of new cap As a result MinC concentration is minimal at the midpoint of the cell, where FtsZ could polymerize
Слайд 47Replication
Timing of cell division
Cell division time could be smaller than
needed for replication of complete genome. Only frequency of replication
initiation determines the doubling time.
