Bashkir State Medical University Department of normal physiology

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1. Bashkir State Medical University Department of normal physiology
2. Employees of the mining industry have constant
3. S15-45%Zn5%As≈3%Cu3,5%Pb0,2%CdCrFeMgOther elementsThe ore
4. Heavy metals content in the body and,
5. One of the pathogenetic links, when the
6. The aim of our research was to
7. Material and methods: The research was carried
8. The kidneys were removed and homogenized for
9. The device HL-003 was used for registration
10. Results and their discussion
11. Note: the reliability of differences in the
12. Record CL homogenate of rats kidneys in
13. ROSOxidative stress
14. Conclusions.The injection of copper-zinc pyrite ore to
15. My personal contribution in this scientific research: 1)
16. Thanks for attention!
17. Скачать презентанцию

Employees of the mining industry have constant contact with the ore, so the problem of intoxication of workers with heavy metals continues to maintain its relevance.

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Bashkir State Medical University Department of normal physiology

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Слайд 1 Bashkir State Medical University Department of normal physiology
Scientific advisors: Ph.

D. in Medicine, full professor A.F. Kayumova, D.Sc. in Medicine,

associate professor O.S. Kiseleva, head teacher K.R. Ziyakaeva

EFFECT OF COPPER-ZINC PYRITE ORE ON THE PROCESSES OF FREE RADICAL OXIDATION IN KIDNEY TISSUE.

Ufa , Russia 2019

The authors: Karaninskii E.V., Mullayanova A.N., Khabibullina I.Z., Famutdinova A.I., Bikbaeva G.B., Nakieva A.G. Biktasheva E.E.

Bashkir State Medical University Department of normal physiology Scientific advisors: Ph. D. in Medicine, full professor

Слайд 2Employees of the mining industry have constant contact with the

ore, so the problem of intoxication of workers with heavy

metals continues to maintain its relevance.

Employees of the mining industry have constant contact with the ore, so the problem of intoxication of

Слайд 3S
15-45%
Zn
5%
As
≈3%
Cu
3,5%
Pb
0,2%
Cd
Cr
Fe
Mg
Other elements
The ore

Слайд 4Heavy metals content in the body and, accordingly, their influence

on different organs and systems in persons who have contact

with ore. The influence of its components launches the pathophysiological process in bone tissue, liver and other organs.

Heavy metals content in the body and, accordingly, their influence on different organs and systems in persons

Слайд 5One of the pathogenetic links, when the body is exposed

by the ore components, is a change in the state

of free radical oxidation (FRO), it is the formation of reactive oxygen species (ROS), which initiate lipid peroxidation (POL). The kidneys are an important organ of elimination, however, the changes occurring in the renal tissue, when it is intoxicated by ore is not enough learned.

Cell

Antioxidant

Free radical

Electron

One of the pathogenetic links, when the body is exposed by the ore components, is a change

Слайд 6The aim of our research was to study the state

of free radical oxidation in the tissue of the kidneys

of rats with copper-zinc pyrite intoxication.

The aim of our research was to study the state of free radical oxidation in the tissue

Слайд 7Material and methods: The research was carried out on 40

outbreeding male rats, 230±15 g weight. They were orally fed

with comminuted into powder copper-zinc pyrite ore, which was gotten from Uchalinsky mining plant. Rats were divided into 4 groups depending on the time of exposure of the ore.
We worked in program "Statistics" on the computer, using Mann Withny method.

Material and methods: The research was carried out on 40 outbreeding male rats, 230±15 g weight. They

Слайд 8The kidneys were removed and homogenized for 5 minutes at

t°- 4°C. The ratio was 1.0 g of tissue per

5 ml of phosphate buffer. Further dilution was carried out in phosphate buffer to a ratio of 1 mg/ml, then 20 ml were taken, which placed in the chamber of the luminomer, and luminescence initiated by the addition of 1 ml of a 50 mM solution of ferrous sulfate was registered.

The kidneys were removed and homogenized for 5 minutes at t°- 4°C. The ratio was 1.0 g

Слайд 9The device HL-003 was used for registration processes of free

radical oxidation arising from the interaction of free radicals.

The device HL-003 was used for registration processes of free radical oxidation arising from the interaction of

Слайд 10Results and their discussion

Слайд 11Note: the reliability of differences in the values of the

experimental groups on the 10th, 20th and 30th day of

the experiment in relation to the control group with * - p≤0,05 and ** - p≤0,01.

Luminescence values in rat kidney homogenate.

Note: the reliability of differences in the values of the experimental groups on the 10th, 20th and

Слайд 12Record CL homogenate of rats kidneys in the control (1)

and experimental groups: for 10 days (2) and 30 days

of research (3).

1.Control group

2.Group 10 days

3.Group 30 days

Record CL homogenate of rats kidneys in the control (1) and experimental groups: for 10 days (2)

Слайд 13ROS
Oxidative stress

Слайд 14Conclusions.
The injection of copper-zinc pyrite ore to rats caused an

increase in the chemiluminescence of the kidney’s homogenate, starting from

the 10th day of the research, remaining at a high level up to 30 days, which testifies to the acceleration of lipid peroxidation processes in the renal tissue.