Study on the effects of water treated with different doses of ultraviolet

properties of micro organisms. Some evidences confirm that low doses

of radiation and changed solar activity cause optimization of cultural activity of a number of microorganisms.
Our investigations were aimed at evaluating the growth properties of microorganisms participating in foods production processes (bread-making, brewing and sour milk products manufacturing) - bifidobacteria, lactobacilli and bakery yeast - in conditions of their culturing on media prepared with the use of water pretreated with hard ultraviolet irradiation.

aureus,
Saccharomyces cerevisiae
The strains, obtained from RAS IMBP RF

SSRC collection were used in the study.  

Strains of microorganisms

the surface of nutrient agar in the form of a

macro colony for 24 hours at 37 °C. After incubation, the macro colony was sterilized with chloroform vapor and covered with semi-liquid agar containing the following cultures:
- Staphylococcus aureus;  
- Pseudomonas aeruginosa (blue pus bacillus);  
- Escherichia coli (colon bacillus);  
The inoculated media were incubated for 24 hours.
After incubation, the antagonistic activity was evaluated based on the diameter of the zone of growth inhibition around the macro colony of the lactobacillus strain being tested.

Study of microbial antagonism in culture media containing UV- treated water

in sterile physiologic salt solution and titrated in test tubes

containing the standard growth medium. Inoculated media were incubated in a thermostat at 37 °C for 24 hours; then, counting of the grown colonies was carried out.

Evaluation of viable cell counts in the colonies of lactobacilli and bifidobacteria grown on the culture media containing water treated with ultraviolet light

surface of 5 % blood agar prepared with the use

of water treated with ultraviolet light. Blood agar prepared with using the ordinary water served as control. The inoculated medium was incubated for 24 hours at 37 °C. After incubation, activity of hemolysin production was evaluated.

Determination of hemolysin activity.

with ultraviolet light (CFU/ml)

UV-treated water treated with ultraviolet

of growth media containing water treated with ultraviolet light (CFU/ampoule).

media containing water treated with ultraviolet light
11

(CFU/ml)

(mm)

directed flux of neutrons (CF/ml)

the whole set of phenotypic features
controlled by the plasmid.
Viable

cell counts for colonies in growth media containing water treated with different methods.

treated with ultraviolet light.

in conditions of prolonged lactobacillus cultures exposure
24

with the use of water treated with ultraviolet light was

not accompanied by changes in the frequency of plasmids transfer or changes in plasmid segregation stability.
The specified features were not reproduced in case of lactobacilli or bifidobacteria passage to ordinary media after exposure in the media containing water treated with ultraviolet light.

Conclusions

use of culture media and biologic objects for implementing the

process being studied can be proposed:
 

A. Sour milk products manufacturing
1. Block of reconstitution of industrial strains of micro organisms from lyophilized condition;
2. Barm preparing block;
3. Sour milk products preparing block.
 

B. Bread-making
1. Block of reconstitution of industrial strains of micro organisms from lyophilized condition;
2. Barm preparing block;
3. Dough preparing block.

industrial strains of micro organisms from lyophilized condition;
2. Beer

yeast preparing block;
3. Beer preparing block.

D. Preparing the inoculum for biological degradation of food products and physiologic waste
1. Block of reconstituting strains and micro organism associations from lyophilized condition;
2. Block of preparing the inoculum;
3. Fermentation block.
 

The following possible installation of the units related to the use of culture media and biologic objects for implementing the process being studied can be proposed: